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TECHSPEC® components are designed, specified, or manufactured by Edmund Optics. Learn More

520nm CWL, 12.5mm Dia, 36nm Bandwidth, OD 6 Fluorescence Filter

Stock #67-016 In Stock
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Diameter (mm):
12.50 +0.00/-0.10
Angle of Incidence (°):
0 ±5
Bandwidth (nm):
OD 6 Blocking Wavelength Range (nm):
250 - 488 & 560 - 700
Optical Density OD (Average):
Clear Aperture CA (mm):
Center Wavelength CWL (nm):
Full Width-Half Max FWHM (nm):
Substrate: Many glass manufacturers offer the same material characteristics under different trade names. Learn More
Fused Silica (Corning 7980)
Minimum Transmission (%):
Hard Coated
Surface Quality:
Thickness Tolerance (mm):
Transmission (%):
Bandpass Filter
Blocking Wavelength Range (nm):
250 -1025
Mounted in Black Anodized Ring
Transmitted Wavefront, RMS:
λ/4 (prior to coating)
Compatible Fluorophore:
GFP Emission
Environmental Durability:
Humidity per MIL-STD-810H, Section 507.6
Temperature per MIL-STD-810H, Section 501.7 and 502.7
Physical Durability:
Adhesion per MIL-PRF-13830B, Section C.4.5.12
Moderate abrasion per MIL-PRF-13830B, Section C.4.5.11
Cleaning per MIL-C-48497A Section
Mount Thickness (mm):
3.5 ±0.1
Substrate Thickness (mm):
2.00 ±0.25

Regulatory Compliance

RoHS 2015:
Certificate of Conformance:
Reach 240:

Product Family Description

Our TECHSPEC® Fluorescence Bandpass Filters are perfectly matched to common fluorophores used in fluorescence imaging applications. Excitation Filters and Emission Filters are available featuring >93% transmission and >OD 6 blocking outside of the passband. These filters are also ideal components in spectroscopy and clinical chemistry applications, as well as biotech instruments such as DNA sequencers and polymerase chain reaction (PCR) testing platforms. Each filter is hard sputtered coated and mounted in a black anodized ring.

For a complete list of compatible fluorophores for each Excitation or Emission Filter, view Fluorophores and Optical Filters for Fluorescence Microscopy.

Excitation filters and emission filters are crucial components in microscopy and imaging applications. Engineered with cutting-edge technology, these filters are designed to enhance signal-to-noise ratios, improve contrast, and deliver unparalleled performance in optical imaging applications. Excitation filters isolate the specific wavelength of light that excites the fluorophores in the sample, enabling them to emit fluorescence. On the other hand, emission filters allow only the emitted fluorescence to pass through while blocking the excitation light, producing a clear and accurate image. These filters are vital in ensuring precise imaging and accurate fluorescence detection in various microscopy techniques. 

These fluorescence bandpass filters offer a range of exceptional features that make them ideal for various fluorescence microscopy systems and imaging techniques. Each filter is manufactured with high-quality materials and state-of-the-art optical coatings, ensuring exceptional spectral performance and minimal light leakage. This results in superior image clarity and accuracy.

Bandpass filters are optimized for maximum transmission within a specific wavelength range, effectively isolating fluorescence signals from background noise, enhancing sensitivity and enabling more accurate detection of fluorophores. The excitation filter design ensures that the filters provide excellent transmission of the desired excitation wavelength, achieving over 93% (typical) transmission efficiency while maintaining a sharp spectral cutoff to minimize the transmission of other wavelengths to less than 0.001%. The emission filters match the performance of excitation filters, with over 93% (typical) transmission of the desired emission wavelength and an equally sharp spectral cutoff, ensuring minimal interference from other wavelengths. Achieve optimal signal intensity with minimal loss of fluorescence signal with these filters' high transmission efficiency. 

These filters are designed to be widely compatible with microscopes from Nikon, Olympus, and Zeiss, making them suitable for a range of imaging applications, including confocal microscopy, widefield fluorescence microscopy, and multiphoton microscopy. Whether you're conducting cellular imaging, live cell imaging, or tissue imaging, our filters are engineered to deliver consistent and reliable results. 

Fluorescence microscopy, immunofluorescence, FRET, FISH, live cell imaging, and high-content screening are common applications of fluorescence imaging. These techniques use fluorescent molecules or dyes to visualize a sample's specific structures, molecules, or DNA sequences. Live cell imaging observes dynamic processes in living cells, while high-content screening screens large numbers of samples for specific features or characteristics.

Whether studying cellular dynamics, protein localization, or molecular interactions, these filters empower sharper, more accurate results confidently. Their robust high-performance design facilitates accurate and efficient fluorescence imaging and contribute to the reliability and repeatability of scientific experiments. Built to withstand the rigors of laboratory use, this ensures long-lasting performance and reliability for research and imaging applications.

 Explore our range of filters today and unleash the full potential of your fluorescence microscopy setup.

All mounted TECHSPEC® Optical Filters have an arrow on the side of the mount that points to the filter-coated surface for quick reference. Filter oriented such that arrow points to filter coated surface S1. Anti-reflective (AR) coating is applied to S2.
All mounted TECHSPEC® Optical Filters have an arrow on the side of the mount that points to the filter-coated surface for quick reference. Filter oriented such that arrow points to filter coated surface S1. Anti-reflective (AR) coating is applied to S2.